Fig. 9.

The liver BAs of Cyp2c70 KO mice are more cytotoxic and resemble a human-like composition. A: The structure, common name, position of the hydroxyl groups, relative hydrophobicity, and hemolysis potential of the individual major BAs commonly found in mammals. Red blood cells isolated from mouse whole blood were incubated with the indicated concentrations of BAs for 30 min at 37°C and the amount of hemolysis was measured. B: Composition and hemolysis profile of liver BAs in female WT and Cyp2c70 KO mice–fed chow or chow plus SC-435 as compared to human. The calculated hydrophobicity index (HI) and compositions of the BA mixtures used for the incubations are shown. C: AML12 (alpha mouse liver 12) cells were incubated with the indicated concentration of BA mixtures modeling the liver BA composition of WT or Cyp2c70 KO mice and cell viability was quantified by MTT assay. D: AML12 cells were incubated with the indicated concentration of BA mixtures and BA toxicity measured by lactate dehydrogenase (LDH) release. Mean values ± SD are shown. BA, bile acid.