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. 2022 Sep 3;14(17):3648. doi: 10.3390/nu14173648

Figure 8.

Figure 8

Inhibitory effect of clusterin and TFF3 macrophage inflammation in RAW264.7 cells (A,B) RAW264.7 cells treated with clusterin or TFF3 (0, 0.5, 5, 10 μg/mL) for 8 h. The cell viability was detected using Cell−Counting−Kit−8 (C) RAW264.7 cells were pretreated with clusterin or TFF3 (5 μg/mL) for 8 h after co−incubation with LPS (1 μg/mL) for 8 h. The TNF−α level was detected using ELISA kits. (D–F) RAW264.7 cells treated with clusterin or TFF3 (5 μg/mL) for 8 h after incubating with LPS (1 μg/mL) for 8 h. The expression of TLR4 was detected using Western blotting. *** p < 0.001 versus the LPS induced group.