FIG. 2.

Northern blot analysis of nfrA transcription. Heat stress was applied. At the indicated times, cells were harvested and RNA was isolated. Equal amounts of RNA were used for the analysis. The positions of the 23S and 16S rRNAs are indicated. (A) RNA from heat-shocked cells. Time after heat shock and growth temperature are indicated. Different bands of 0.8 and 2 kb are visible. (B) RNA from puromycin-treated cells. Puromycin (20 μg/ml) (+) was added to the cell suspension; −, no puromycin. Cells were harvested at the indicated times, and RNA was isolated.