FIG. 6.

Comparison of nfrA and ywcH induction. (A) Induction of transcriptional fusions of lacZ to the respective gene during growth in NB supplemented with glucose and glutamate. Filled circles, growth of B. subtilis IPA43, given as OD₆₀₀ units; open circles, β-galactosidase (β-gal) activity of B. subtilis IPA43 (nfrA-lacZ fusion in nfrA); squares, β-galactosidase activity of B. subtilis IPA44 (ywcH-lacZ fusion in ywcH); inverted triangles, β-galactosidase activity of B. subtilis IPA44E (ywcH-lacZ fusion in amyE); triangles, β-galactosidase activity of B. subtilis DH32M (control). (B) Heat shock induction of nfrA and ywcH. Filled symbols, activity in cells grown at 37°C; empty symbols, activity of stressed cells; circles, B. subtilis DIPA4 (nfrA-bgaB); squares, B. subtilis DIPA44 (ywcH-bgaB).