TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant markers | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | hsdR17 endA1 recA1 gyrA96 thi relA1 supE44 φ80dlacZΔM15 Δ(lacZ-argF)U169 | Bethesda Research Laboratories |
| B. subtilis 168 | trpC2 | Bacillus Genetic Stock Center |
| B. subtilis DL | trpC2 amyE::(cat bgaB) | 34 |
| B. subtilis DH32M | trpC2 amyE::(cat spoVG::lacZ) | 34 |
| B. subtilis DIPA4 | trpC2 amyE::(cat nfrA-bgaB) | 34 |
| B. subtilis DXYL strains | trpC2 amyE::(cat PnfrA-PxylA–bgaB) | This work |
| B. subtilis B49 | trpC2 amyE::(cat nfrA-bgaB) | This work |
| B. subtilis DIPA4mut | trpC2 amyE::(cat nfrA-bgaB) | This work |
| B. subtilis IPA43 | trpC2 cat nfrA-spoVG::lacZ | This work |
| B. subtilis IPA44 | trpC2 cat ywcH-spoVG::lacZ | This work |
| B. subtilis IPA44E | trpC2 amyE::(cat nfrA-spoVG::lacZ) | This work |
| B. subtilis DIPA44 | trpC2 cat ywcH-bgaB | This work |
| Plasmids | ||
| pDL | bla amyE::(cat bgaB) | 57 |
| pIC20H | bla | 31 |
| pDH32M | bla cat spoVG::lacZ | 24 |
| pMUTIN2 | bla cat spoVG::lacZ | 49 |
| pKL4 | bla cat bgaB | 42 |
| pIPA6 | bla cat nfrA-ywcH | 34 |
| pIPA8 | bla nfrA | This work |
| pIPA11 | bla erm nfrA | 34 |
| pIPA14 | bla ywcH | This work |
| pDIPA4 | bla amyE::(cat nfrA-bgaB) | 34 |
| pB49 | bla amyE::(cat nfrA-bgaB) | This work |
| pDIPA4mut | bla amyE::(cat nfrA-bgaB) | This work |
| pDXYL plasmids | bla amyE::(cat PnfrA-PxylA–bgaB) | This work |
| pIPA43 | bla cat nfrA-spoVG::lacZ | This work |
| pIPA44 | bla cat ywcH-spoVG::lacZ | This work |
| pIPA44E | bla cat nfrA-spoVG::lacZ | This work |
| pDIPA44 | bla cat ywcH-bgaB | This work |