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(A) Time-dependent covalent modification of wildtype K-Ras and CysLight K-Ras(G12R) by compound 3 (50 μM). (B) Reaction between K-Ras mutants and compound 3 (50 μM, 16 h). (C) Reaction between K-Ras(G12R)·GDP and compound 3 (50 μM) at various pH. (D) Differential scanning fluorimetry of K-Ras(G12R)·GDP and K-Ras(G12R)·GDP·3 adduct.
