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Schematic of maternal diet and drug regimens and tissue collection time points. Dams were fed a chow (CH) or high-fat (HF) diet and given water (CT) or metformin (MT) throughout gestation and lactation. Offspring were all weaned on a chow diet and given water without metformin. Tissues, plasma and colonic contents were collected at postnatal day (PND) 21 and 16 weeks of age separately.
