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. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: Nat Protoc. 2021 Mar 1;16(4):1802–1829. doi: 10.1038/s41596-020-00476-z

Figure 1. Differentiation of hPSC to AFE.

Figure 1.

A. Schematic representation of the differentiation protocol until day 6. B-E. Bright-field views after culture of RUES2 ESC to confluence (B), to DE (C) and to AFE (D,E) (representative of n>10 independent experiments). F. Representative example of the flow cytometric profile after staining day 4 RUES2 ESC and sviPSC DE cells for CXCR4 and cKIT (representative of n>10 independent experiments). DE, definitive endoderm; AFE, anterior foregut endoderm.