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. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: Nat Protoc. 2021 Mar 1;16(4):1802–1829. doi: 10.1038/s41596-020-00476-z

Figure 5. Differentiation of hiPSC-derived lung progenitors to airway and distal cells at day 50 of the differentiation protocol.

Figure 5.

A. Schematic representation of the differentiation protocol until day 50. B. Bright-field view after culture of sviPSC to day 50 of the differentiation protocol (representative of n>10 independent experiments, scale bar: 500μm). C-J. Representative examples of the expression of mature markers of distal and airway epithelial cells after culturing sviPSC to day 50 according to the protocol shown in A (representative of n>5 independent experiments, inset scale bar: 20μm). DE, definitive endoderm; AFE, anterior foregut endoderm; LP, lung progenitor.