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. Author manuscript; available in PMC: 2022 Sep 9.
Published in final edited form as: Nat Protoc. 2021 Mar 1;16(4):1802–1829. doi: 10.1038/s41596-020-00476-z

Figure 6. Extension of the maturation period yields more mature basal cells.

Figure 6.

A. Schematic representation of the differentiation protocol until day 80. B. Representative examples of the expression of mature markers of basal cells, NGFR and KRT14, after culturing RUES2 ESC to day 80 according to the protocol shown in A (representative of n=6 independent experiments, inset scale bar: 20μM). DE, definitive endoderm; AFE, anterior foregut endoderm; LP, lung progenitor.