TABLE 3.
In vivo competition assays with wild-type serovar Typhimurium and isogenic mutants
| Mutant | Route of inoculationa | Median CIb | No. of mice | Pc |
|---|---|---|---|---|
| gipA2::MudJ | Oral | 0.45 | 16 | <0.05 |
| i.p. | 2.32 | 9 | NS | |
| Oral (Peyer's patch)d | 0.10 | 5 | <0.0005 | |
| gipA2::MudJ/pJS203 | Oral (Peyer's patch)d | 0.59 | 5 | NS |
| gfoJ::MudJ | Oral | 1.5 | 5 | NS |
| ΔgfoA::kan | Oral | 1.3 | 5 | NS |
| ΔGifsy-1::kan | Oral | 0.28 | 12 | <0.00005 |
a
All inocula contained a 1:1 ratio of mutant to isogenic wild-type serovar Typhimurium (JS135). Doses given to BALB/c mice were 102 CFU i.p. and 108 CFU orally. Bacteria were recovered from the small intestine after oral infection or the spleen after i.p. infection unless otherwise indicated.
b
Defined as output (mutant/wild type) divided by input (mutant/wild type).
c
Student's t test was used to compare output versus inoculum. NS, not significant.
d
Bacteria were recovered from individual dissected Peyer's patches after oral infection.