TABLE 1.

Oligonucleotides used in this work^a

Primer	Sequence (5′→3′)
1	5′-GGATGTTCATATGAAACTTGACCAGATT-3′ (NdeI)
2	5′-GCGGGGATCCCTAACCGCGCCCGTTTTTCGT-3′ (BamHI)
3	5′-GCGGGATCCCTGCCCAATATTTAAGCG-3′ (BamHI)
4	5′-CTCGGATCCATTCAGATCAATCTGGTC-3′ (BamHI)
5	5′-GCGTGGATCCCAATCCGGGGGGGCATATG-3′ (BamHI)
6	5′-GTTTTTTCTGGTCGACCTC-3′
7	5′-GATCGGCGTGTGCAGCAT-3′
8	5′-CAAGCTGTCTTACCCGTT-3′
9	5′-GGATTAAGAGTGACATTCC-3′
10	5′-CTGCCTAATTCCCTCATC-3′
11	5′-AGCGGCCATTTGACAGAG-3′
12	5′-GGCCGGATCCTACAGTTCCCTAGAAAAG-3′ (BamHI)
13	5′-GGCCGGATCCATCGTGCGAAGTCTTCGG-3′ (BamHI)
14	5′-GGCCGGATCCTTTGATTTTGTACACATT-3′ (BamHI)
15	5′-GGCCGGATCCCCGAACATTTTTTGAAAG-3′ (BamHI)
16	5′-GGCCGGATCCATGCCCCCCCGGATTGTT-3′ (BamHI)
17	5′-GGCCGGATCCTTGTCATTTACAAAAATACCC-3′ (BamHI)
18	5′-GGCCGAATTCCTGCCCAATATTTAAGCG-3′ (EcoRI)
19	5′-GCGTGAATTCCAATCCGGGGGGGCATATG-3′ (EcoRI)
20	5′-GCGATTAAGTTGGGTA-3′
21	5′-CCAAGCTTATCGCGTGGCTGACAAAG-3′
22	5′-TCTTCCCGATGATTAAGCTTG-3′
23	5′-TTCTGCCTAATTCCCTCATC-3′

^aSome of these oligonucleotides were engineered to contain NdeI, BamHI, and EcoRI restriction sites (underlined) at the 5′ end. In primers 1 and 2, used to clone the lrpC gene in overexpressing vector pET3b (36), translation initiation and termination codons are in bold.