Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 8;13:465. doi: 10.1186/s13287-022-03157-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — The IFN-γ- and KYNA-primed hADSCs significantly inhibited ECM deposition and EMT process. (A) Collagen staining analysis for therapeutic efficacy evaluation of colon fibrosis in rats; Up: Sirius Red staining; Bottom: Masson’s trichrome staining and Staining area statistics by the ImageJ software (n = 5); (B) qPCR analysis of fibrosis-related gene expression (Acta-2, TGF-β1, Timp-1, MMP-2, and MMP-9) in the colon on 46th day after treatment (n = 5); (C) Western blot analysis of laminin, vimentin, and α-SMA protein expression; (D) Immunofluorescence staining of the epithelial marker E-cad and myofibroblasts marker α-SMA in frozen sections of the colon (Scale bar, 100 μm); (E) Immunofluorescence staining of the epithelial marker E-cad and stromal marker vimentin in frozen sections of the colon (Scale bar, 100 μm); Data are represented as mean ± SEM