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. 2022 Aug 26;10:949724. doi: 10.3389/fbioe.2022.949724

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Copyright © 2022 Gu, Yin, Song, Wu, He, Zhu, Wu, Zhao, Huang, Wang, Tong and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Safety evaluation of hiPSC-exosomes at the cellular level. (A) Absorbance values of various groups of erythrocyte suspensions at 540 nm. N = 4 per group (ns, no statistical difference; ****p < 0.0001). (B) Fluorescence image of DNA after alkaline comet electrophoresis of leukocytes in PBS, hiPSC-exosomes, and the 50 μM camptothecin group. (C) Tail length analysis of comets in the three groups. N = 5 per group (*p < 0.05). (D) PKH26-labeled hiPSC-exosome uptake by RAW264.7 cells under laser scanning confocal microscopy. (E) Viability of RAW264.7 cells treated with up to 200 μg/ml hiPSC-exosomes. N = 4 per group. Data are expressed as the mean ± SEM.