Fig. 3.

Targeting microglial miR-155 ameliorates inflammation in APP/PS1 mouse retinas. a–c Densitometric analyses of western blotting protein bands of a phosphorylated NF-κB p65, b mature IL-1β, and c. TNF-α normalized by a total NF-κB p65 or b and c β-actin control for retinal lysates from all experimental groups (n = 48 total, n = 6 each group). d–i Meso Scale Discovery analysis for protein expression of cytokines including d IL-2, e IL-5, f IL-12, g IFN-γ, h IL-6 and i IL-10 from the same mouse cohort. Data from individual mice (circles) as well as group means ± SEMs are shown. Black-filled circles represent male and clear circles represent female animals. *p < 0.05, **p < 0.01, by three-way ANOVA with Tukey’s post-hoc multiple comparison test. Two group statistical analysis was performed using an unpaired two-tailed Student t-test and is shown in parentheses. Fold changes and percentage decreases are shown in red