Table 1.
List of antibodies
| Antibody or reagent | Source species | Dilution | Application | Source | Catalog. # |
|---|---|---|---|---|---|
| Primary antibody | |||||
| Fcrls mAb | Rat | 1:200 | FCM | Butovsky Lab | N/A |
| CD11b-PeCy7 | Rat | 1:300 | FCM | eBioscience | 50-154-54 |
| Ly-6C-PerCP/Cy5.5 | Rat | 1:300 | FCM | BioLegend | 123,012 |
| Clec7a mAb | Rat |
1:100 1:50 |
IF-CS IF-FL |
InvivoGen | Mabg-mdect |
| TMEM119 mAb | Rabbit |
1:500 1:200 |
IF-CS IF-FL |
Synaptic Systems | 400,011 |
| Alexa Fluor 488-conjugated tomato lectin | Lycopersicon esculentum |
1:200 1:100 1:100 |
IF-CS IF-FL IF-RMVI |
DyLight | DL-1174 |
| Galectin-3 pAb | Goat |
1:100 1:50 1:1000 |
IF-CS IF-FL WB |
R&D Systems | AF1197 |
| IBA1 | Rabbit |
1:100 1:50 |
IF-CS IF-FL |
Fujifilm Wako Chemicals | 019-19,741 |
| IBA1 | Goat |
1:100 1:50 1:1000 |
IF-CS IF-FL WB |
Novus Biologicals | NB100-1028 |
| Aβ42 (12F4) mAb | Mouse | 1:200 | IF-CS | BioLegend | 805,501 |
| P2ry12 | Rabbit |
1:200 1:500 |
IF-CS WB |
Butovsky Lab | N/A |
| APOE pAb | Goat | 1:200 | IF-CS | Millipore Sigma | AB947 |
| phospho-NF-κB p65 Ser536 mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 3033S |
| NF-κB p65 mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 8242S |
| TNF-α mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 11948S |
| IL-1β mAb | Mouse | 1:1000 | WB | Cell Signaling Technology | 12242S |
| EIF3c pAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 2068S |
| NDUFA6 pAb | Rabbit | 1:1000 | WB | Thermo Fisher Scientific | PA5-97,259 |
| NDUFA10 pAb | Rabbit | 1:1000 | WB | Thermo Fisher Scientific | PA5-21,474 |
| Spp1 | Goat |
1:100 1:20 1:500 |
IF-CS IF-FL WB |
R&D Systems | AF808 |
| GFAP | Rat | 1:50 | IF-FL | Invitrogen | 13–0300 |
| 4G8 mAb | Mouse | 1:200 | IF- RMVI | Biolegend | 800,701 |
| Aβ40 (11A50-B10) mAb | Mouse | 1:200 | IF- RMVI | Biolegend | 805,401 |
| Zonula Occluden-1 pAb | Rabbit | 1:50 | IF- RMVI | Thermo Fisher Scientific | 61–7300 |
| Claudin-1 pAb | Rabbit |
1:75 1:1000 |
IF- RMVI WB |
Thermo Fisher Scientific | 51–9000 |
| MMP-9 | Goat | 1:800 | WB | R&D Systems | AF909 |
| Secondary antibody | |||||
| APC anti-rat IgG clone Poly4054 | Goat | 1:300 | FCM | BioLegend | |
| Cy2 (anti-Rabbit, anti-goat, anti-rat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
| Cy3 (anti-rabbit, anti-goat, anti-rat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
| Cy5 (anti-mouse, anti-rabbit, anti-rat, anti-goat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
| Peroxidase Donkey Anti-Rabbit IgG (H + L) | Donkey | 1:10,000 | WB | Jackson ImmunoResearch Laboratories | |
| Peroxidase Donkey Anti-Mouse IgG (H + L) | Donkey | 1:10,000 | WB | Jackson ImmunoResearch Laboratories | |
| Peroxidase Donkey Anti-Goat IgG (H + L) | Donkey | 1:10,000 | WB | Jackson ImmunoResearch Laboratories | |
FCM Flow cytometry, IF immunofluorescence, CS cross-section, FL flat mount, RMVI retinal microvascular isolation, WB western blot, pAb polyclonal antibody, mAb monoclonal antibody, N/A not applicable