Table 1.

Comparison of the binding affinities (Kd, n = 3) of mAbs to human 4-1BB

Antibody	Kon (1/Ms)	Koff (1/s)	Kd (nM, SPR)	Kd (nM, BLI)	4-1BBL competition
Urelumab	209,700	0.0011	5.34 ± 0.49	5.76 ± 0.85	No
Utomilumab	504,900	0.0089	17.69 ± 1.92	22.12 ± 3.75	Yes
HuB6	94,260	0.0018	18.56 ± 2.49	13.98 ± 2.60	Yes

Yes/No indicates whether 4-1BBL can compete for binding to 4-1BB