TABLE 1.
B. subtilis strains
| Strain | Relevant characteristics | Origin or reference |
|---|---|---|
| BR151 | trpC2 metB10 lys-3 | Laboratory stock |
| ZB307 | SPβc2Δ2::Tn917pSK10Δ6 | P. Youngman (26, 30) |
| SL7205 | SPβc2Δ2::Tn917pSK10Δ6 amyE::spoIIR-gusA spoIIR::spoIIR-lacZ | This study |
| SL7213 | amyE::spoIIE-gusA SPβc2Δ2::Tn917pSK10Δ6::spoIIE-lacZ | This study |
| SL7256 | SPβc2Δ2::Tn917pSK10Δ6::spoIIR spoIIR::neo | This study |
| SL7258 | SPβc2Δ2::Tn917pSK10Δ6 spoIIR::neo amyE::spoIIR | This study |
| SL7303 | amyE::spoIIR-gusA SPβc2Δ2::Tn917pSK10Δ6::spoIIR-lacZ | This study |
| SL7304 | amyE::spoIIR-gusA SPβc2Δ2::Tn917pSK10Δ6::spoIIR-lacZ spoIIAC561 | This study |
| SL7310 | trpC2 metB10 lys-3 amyE::IIR-gusA thrC::spoIIR-lacZ | This study |
| SL7313 | trpC2 metB10 lys-3 spoIIR::neo thrC::spoIIR | This study |
| SL7321 | SPβc2Δ2::Tn917pSK10Δ6 spoIID-lacZ@spoIIDa | This study |
| SL7322 | SPβc2Δ2::Tn917pSK10Δ6::spoIIR spoIIR::neo spoIID-lacZ@spoIID | This study |
| SL7340 | trpC2 metB10 lys-3 spoIIR::neo amyE::spoIIR | This study |
| SL7344 | trpC2 metB10 spoIIR::neo SPβc2Δ2::Tn917pSK10Δ6::spoIIR | This study |
| SL8344 | trpC2 metB10 lys-3 spoIIQ-gfp | This study |
| SL8345 | trpC2 metB10 lys-3 spoIIR::neo spoIIQ-gfp | This study |
| SL8378 | trpC2 metB10 lys-3 spoIIR::neo amyE::spoIIR spoIIQ-gfp | This study |
| SL8380 | trpC2 metB10 lys-3 spoIIR::neo SPβc2Δ2::Tn917pSK10Δ6::spoIIR spoIIQ-gfp | This study |
| SN178 | SPβc2Δ2::Tn917pSK10Δ6 amyE::spoIIR-gusA spoIIR-lacZ@spoIIR spoIIAC561 | This study |
a
@ indicates the fusion is located at the locus by a Campbell-like recombination.