TABLE 3.
Frequency of monosporic and disporic phenotypes in strains having spoIIR at different chromosomal locationsa
| Strain | Location of intact copy of spoIIR | No. of organisms with expression pattern of spoIIQ-gfp
|
|
|---|---|---|---|
| Disporic | Monosporic | ||
| SL8344 | spoIIR | 0 | 200 |
| SL8378 | amyE | 1 | 199 |
| SL8380 | SPβ | 54 | 146 |
| SL8345 | None | 61 | 139 |
a
The phenotypes were assayed for each strain by scoring 200 organisms that were displaying fluorescence from GFP resulting from prespore-specific expression of a spoIIQ-gfp transcriptional fusion (12). Organisms were sampled 6 h after the end of exponential growth at 33.5°C in MSSM; similar results were obtained with samples taken at 5 and 8 h (data not shown). The strains are in the BR151 genetic background.