Fig. 5.

Evaluation of the MSC-like properties in CLP single cell-derived clones. a Diagram of the strategy used to obtain single cell clones. b qPCR analysis for the expression of the MSC-surface markers CD106 and CD146 in BM-MSCs, the parental CLP and 4 representative clones (10, 20, 29 and 32). In the table to the right comparable or gained expression of these markers in the clones when compared to CLP is reported. *p < 0.05 BM-MSCs versus CLP and clones; °p < 0.05 CLP versus clones. c qPCR data were similarly confirmed by FACS analysis of CD106 and CD146 in the same groups. Gating strategy and histograms are presented in Additional file 1: Fig. S6. *p < 0.05 BM-MSCs versus CLP and clones; °p < 0.05 CLP versus clones. d Brightfield images after ARS (day 21) and CV staining (day 14) show mineralization and single-cell derived colonies in BM-MSCs, CLP and Clones 10, 20, 29 and 32. Histograms to the right report quantification of the ARS staining by ImageJ and quantification of the colonies. Scale bars: 5 µm (ARS); 1 cm (CV). *p < 0.05. a.u.: arbitrary unit