Table 3.
Detection of SARS-CoV-2 and SARS-CoV-2 nucleotide substituted RNA in WWTP samples by HT-qPCR.
| Date of sample collection | SARS-CoV-2 RNA |
SARS-CoV-2 nucleotide substituted RNA |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CDC N1 | CDC N2 | CDC N1 + N2 | NIID | N_ Sarbeco | D80A | E484K | E484Q | K417N | N501Y | L452R | S69-70 del | T19R | T478K | |
| 22-Mar | −/− | −/+ | −/+ | −/− | +/− | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 29-Mar | −/− | −/− | −/− | −/− | −/+ | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 5-Apr | +/+ | +/− | +/− | −/− | −/+ | NT | −/+ | NT | NT | −/− | −/+ | −/− | NT | NT |
| 12-Apr | −/− | −/− | −/− | −/− | −/− | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 19-Apr | −/− | −/− | −/− | −/− | −/− | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 26-Apr | −/+ | −/+ | −/+ | −/+ | −/+ | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 4-May | −/+ | +/− | +/− | +/− | −/+ | NT | −/− | NT | NT | −/− | −/− | +/− | NT | NT |
| 10-May | −/− | −/− | −/− | −/− | −/+ | NT | −/− | NT | NT | +/− | −/− | −/− | NT | NT |
| 18-May | −/− | −/− | −/− | −/− | −/− | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 24-May | −/− | +/− | +/− | +/− | −/− | NT | −/− | NT | NT | +/− | −/− | +/− | NT | NT |
| 31-May | −/− | −/− | +/− | −/− | −/− | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 7-Jun | −/− | −/− | −/− | −/− | −/− | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 14-Jun | −/− | −/− | −/− | −/− | −/+ | NT | −/− | NT | NT | −/− | −/− | −/− | NT | NT |
| 21-Jun | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 28-Jun | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 5-Jul | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 12-Jul | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 19-Jul | +/− | +/− | +/− | +/− | +/− | −/− | −/− | −/− | −/− | −/− | +/− | +/− | −/− | −/− |
| 26-Jul | −/+ | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 2-Aug | −/+ | −/− | −/+ | −/− | −/− | −/− | −/− | −/− | −/− | −/− | +/− | −/− | −/− | −/− |
| 9-Aug | +/− | +/+ | +/− | +/− | +/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 16-Aug | −/+ | +/+ | −/+ | −/+ | −/− | +/− | −/− | −/− | −/− | −/− | −/− | −/− | +/− | −/− |
| 23-Aug | −/+ | −/+ | −/+ | −/+ | +/+ | −/− | −/− | −/+ | −/+ | −/− | −/− | −/+ | −/+ | −/− |
| No. of positive samples | 3/7 | 6/5 | 6/5 | 4/3 | 4/7 | 1/0 | 0/1 | 0/1 | 0/1 | 2/0 | 2/1 | 3/1 | 1/1 | 0/0 |
+, detected; −, not detected; NT, not tested.
Detection of SARS-CoV-2 and SARS-CoV-2 nucleotide substituted RNA in the combined and separated sewer lines is designated by ‘/’ (combined/separated sewer lines).