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. 2022 Aug 26;13:927641. doi: 10.3389/fphar.2022.927641

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Copyright © 2022 Liang, Liu, Qu, Wang, Zhou, Liang, Guo and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of ferroptosis in the renal tubules of mice at 12, 24, 48, and 72 h after I/R (A) Transmission electron microscopy was used to observe the mitochondria of renal tubules (B) The numbers of ferroptotic mitochondria per field in different groups were compared (C) Western blotting detection of GXP4 and 4-HNE in the renal tissue (D) Protein synthesis of GXP4 and 4-HNE in western blotting. Due to the death of mice, N = 5 for each group. Statistical significance is denoted as *: p < 0.05, **: p < 0.01, ****: p < 0.0001 compared with the sham group at the same time point.