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. 2022 Aug 26;13:927641. doi: 10.3389/fphar.2022.927641

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Copyright © 2022 Liang, Liu, Qu, Wang, Zhou, Liang, Guo and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of ferroptosis in H/R HK-2 cells at 6, 12, and 24 h after H/R (A) Transmission electron microscopy was used to observe the mitochondrion of HK-2 cells (B) The number of ferroptotic mitochondria per field in different groups were compared, the number was highest at 12 h (p < 0.0001) (C) Representative western blotting of GXP4 and 4-HNE, β-actin used as an internal control (D) Protein synthesis of GXP4 and 4-HNE in western blotting (E) Flow cytometry showed that the level of reactive oxygen species (ROS) in the control group and H/R HK-2 cells (F) Analysis of ROS in flow cytometry in H/R HK-2 cells (G) mRNA expressions of GXP4 in qRT-PCR. Statistical significance is denoted as *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001 compared with the control group at the same time point.