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DNase protection assay of the binding of UhpA (open symbols) and P-UhpA (closed symbols) to the strong site of the wild-type and Δ22 uhpT promoters. Occupancy of the strong-site residues that are cleaved by DNase I in the wild-type (circles) and Δ22 variant (squares) promoter fragments is shown. The DNase protection assay is carried out as described for Fig. 1.
