TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| IST27N | Alg−, spontaneous variant of a mucoid CF isolate | 16 |
| 8858 | Alg−, algC mutant | 34 |
| E. coli | ||
| XL1-Blue | recA1 lac[F′ proAB lacIqZΔM15] | 10 |
| HB101 | recA hsdS20(rB− mB−) | 27 |
| JM83 | ara Δ(lac-proAB) rpsL thi φ80 dlacZ ΔM15 | 32 |
| GPM83 | JM83 glmM::kan(pGMM) | 21 |
| PAE831 | JM83 glmM::kan(pMLD136) | This study |
| PAE832 | JM83 glmM::kan(pMLD137) | This study |
| Plasmids | ||
| pCR2.1 | ColE1 Apr Kmr; TA PCR cloning vector | Invitrogen |
| pRK2013 | Kmr Tra+ | 6 |
| pMMB66(HE) | Apr; broad-host-range plasmid | 7 |
| pNZ49 | P. aeruginosa algC in pMMB66(HE) | 34 |
| pIT351 | P. aeruginosa glmM in pCR2.1 | This study |
| pIT352 | P. aeruginosa glmM in pMMB66(HE) | This study |
| pTrc99A | Apr; expression vector | Pharmacia |
| pTrcHis60 | Apr; derivative of pTrc99A | 24 |
| pMLD136 | P. aeruginosa glmM in pTrc99A | This study |
| pMLD137 | P. aeruginosa glmM in pTrcHis60 | This study |