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Phosphoglucosamine mutase activity in E. coli cells expressing the P. aeruginosa glmM gene
| Enzyme source | Growth temp (°C) | IPTG (1 mM) | Sp act (μmol · min−1 · mg of protein−1) |
|---|---|---|---|
| Crude extracts from: | |||
| JM83 | 30, 37, or 42 | − | 0.04 |
| GPM83 | 30 | − | 0.08 |
| 42 | − | 0.001 | |
| PAE831 | 37 | − | 0.008 |
| 37 | + | 0.35 | |
| PAE832 | 37 | − | 0.007 |
| 37 | + | 0.30 | |
| Pure P. aeruginosa GlmM enzyme (His6-tagged form) | 2.5 |
