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. 2022 Aug 29;18(14):5489–5502. doi: 10.7150/ijbs.72977

Figure 2.

Figure 2

Identification of optimal AANG formula for treatment of db/db mice. (A-F) in vitro cytotoxicity assay by MMT. Cultured murine renal tubular epithelial cells mTEC (A-C) and mesangial cells mMES (D-F) were treated with AA, NG, or the combination with indicated dose, the cell proliferation rates were detected by MTT assay at 24h in vitro. (G-K) The db/m or db/db mice received the combination of AA and NG with indicated doses daily from rodent food for 20 weeks since 4-week-old for measuring the serum levels of LDH, ALT, AST, fasting blood glucose and UACR. Data represented mean ± s.d. from groups of 5 mice. (A-F) *p<0.05, **p<0.01 vs control; (G-K) ***p<0.001 vs db/m; #p<0.05, ##<0.01, ###p<0.001 vs db/db; ^p<0.05, ^^^p<0.001 vs db/db+AA.