Preventive treatment with AANG effectively ameliorates the T2D-associated kidney injury and metabolic abnormalities in db/db mice. (A) The 20-week-preventive treatment with an optimal dose of AANG (300mg/kg/day AA and 3000mg/kg/day NG) from week 4 to week 24) effectively improves the renal dysfunction of db/db mice by significantly reducing UACR. (B) Unexpectedly, AANG preventive treatment also markedly normalizes the diabetic index HbA1c level of db/db mice to be insignificant with db/m mice. In addition, AANG preventive treatment not only improves the (C) insulin sensitivity (IPITT) and (D) glucose tolerance (IPGTT), but also reduces the (E) body weight and (F) fasting blood glucose levels in db/db mice. Data represented mean ± s.d. from groups of 6 mice. **p<0.01, ***p < 0.001 vs control db/m mice; #p<0.05, ##p<0.01 vs control db/db mice.