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. 2022 Aug 29;18(14):5489–5502. doi: 10.7150/ijbs.72977

Figure 5.

Figure 5

AANG shows a better preventive effect on rebalancing Smad3/7 and inhibiting NF-κB signaling as well as lncRNA Erbb4-IR and LRNA9884 expression in the diabetic kidney of db/db mice at 24 weeks. (A-D) Real-time PCR analysis shows that compared to AA or AG monotherapy, AANG effectively increases renal expression of anti-inflammatory Smad7 mRNA while inhibiting Smad3, which is associated with inhibition of renal fibrosis including α-SMA and collagen I mRNA expression. (E, F) Real-time PCR detects that preventive treatment with AANG produces a better inhibitory effect on NF-κB activation and MCP-1 mRNA expression. (G, H) Effects of AANG preventive treatment on Smad3-dependent lncRNAs Erbb4-IR and LRNA9884 in the diabetic kidney of db/db mice in 24 weeks. Data represented mean ± s.d. from groups of 6 mice. *p<0.05, **p < 0.01, ***p<0.001 vs db/m mice; #p<0.05, ##p<0.01. ###p<0.001 vs control db/db mice; ^p<0.05, ^^p < 0.01, ^^^p<0.001 vs AA-treated db/db mice.