Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 15;18(14):5260–5275. doi: 10.7150/ijbs.73890

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

Celastrol inhibited the nuclear translocation and interaction of PKM2 and HIF-1α while promoted phenotypic switch of macrophage polarization. (A) Immunofluorescence imaging of cellular PKM2. After treated celastrol and LPS as stated in “Methods”, RAW264.7 cells were analyzed by immunofluorescence staining. Scale bar: 20 µm. (B) WB analysis of cytosolic and nuclear proteins (PKM2 and HIF-1α). (C) Quantitative analysis for the cytosolic and nuclear proteins. (D) Immunoprecipitation of PKM2-HIF-1α complex. Following drug treatment, RAW264.7 cells were lysed and subjected to immunoprecipitation with anti-PKM2 antibody, the bound proteins were analyzed by immunoblotting with anti-HIF-1α antibody. Input PKM2 in each group was immunoblotted as control. (E) Flow cytometric analysis of M1 macrophage biomarker CD86 and M2 macrophage biomarker CD206. ***p < 0.001 (LPS vs others).