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. 2022 Aug 29;18(14):5459–5474. doi: 10.7150/ijbs.74546

Figure 7.

Figure 7

PCBP2 stabilizes SLC7A11 mRNA and the PHGDH+SLC7A11 score can assess BCa prognosis. (A) A qPCR technique was implemented for detecting the SLC7A11 expression after the knock-down of PCBP2. (B) RIP assay using anti-PCBP2 was used to assess the enrichment of SLC7A11 mRNA by PCBP2. IgG was regarded as the Negative control in these experiments. (C) Actinomycin D assay was used to detect the degradation of SLC7A11 mRNA. (D-E) A T24 rescue cell line was constructed by over-expressing PCBP2 in PHGDH knock-down cells, and the SLC7A11 expression was verified using the western blot and qPCR. (F-G) Colony formation and CCK-8 assays were employed for assessing the viability of the cells in the rescue cell lines. (H) Representative IHC images of SLC7A11 in patients with BCa. Scale bars: 50/25 μm. (I) KM survival analysis of patients with BCa according to PHGDH+SLC7A11 score. (J-K) Univariate and multivariate Cox regression analysis of prognostic factors for OS (J) and DFS (K). (L) A nomogram based on PHGDH+SLC7A11 score. (M) The ROC curve of nomogram and TNM staging system for the prediction of OS after 5 years. *p<0.05, **p<0.01, ***p<0.001.