Skip to main content
. 2022 Aug 29;18(14):5522–5538. doi: 10.7150/ijbs.72211

Figure 5.

Figure 5

Ctsk-/-/Mmp9-/- osteoclasts exhibited low Cebpa expression and high Nfatc1 expression, and Ctsk is co-localized to the cell nucleus along with c-Fos. (a) Western blots for important transcriptional factors in osteoclast at the end of 4-day osteoclastogenesis induction, normalized to GAPDH. (b) Quantification of A (n=3 independent experiments). (c) qPCR to assess mRNA expression of important transcriptional factors in osteoclast formation and function at the end of 4-day osteoclastogenesis induction, normalized to Hprt (n=3 independent experiments). (d) Immunofluorescent staining (IF) of c-Fos (green) in addition to Ctsk (red) in mouse femur. Merged image (yellow) indicated co-localization of the proteins (n=3 independent experiments). All the data are presented as mean values ± SD; two-tailed unpaired t test; ns. non-significant.