TABLE 6.
Difference in mRNA levels between the wild-type and resDE mutant strains after anaerobic induction in the absence of nitrate and nitritea
| DNA | Difference in mRNA level (fold)b |
|---|---|
| narG | 37.9 |
| narH | 53.0 |
| narJ | 50.4 |
| narI | 41.4 |
| ywiC | 2.3 |
| ywiD | 4.8 |
| narK | 6.4 |
| fnr | 8.5 |
| nasD | 27.7 |
| nasE | 6.5 |
| nasF | 7.9 |
| hmp | 40.5 |
| cydA | 0.8 |
| cydB | 0.8 |
| cydC | 0.8 |
| cydD | 0.7 |
| ctaA | 19.6 |
| ctaB | 3.8 |
| ctaC | 3.2 |
| qoxA | 4.5 |
| qoxB | 4.1 |
| qoxC | 6.5 |
| qoxD | 3.5 |
| resA | 6.3 |
| resB | 8.4 |
| resC | 5.4 |
| hemE | 7.5 |
| hemH | 8.1 |
| hemY | 5.3 |
| lctE | 3.9 |
| alsS | 5.5 |
| alsD | 5.8 |
| sunA | 3.1 |
| yolI | 4.0 |
| yolJ | 3.7 |
| sbo | 9.9 |
| yfmQ | 15.5 |
| ytkA | 12.3 |
| dps | 7.6 |
| ywfI | 4.4 |
| ywcJ | 18.3 |
| yxaK | 4.4 |
| yxaL | 5.1 |
| yncM | 6.1 |
| yvyD | 6.8 |
| yckF | 5.1 |
| yckG | 5.1 |
| yjdD | 19.8 |
| yjdE | 6.8 |
| yjdF | 2.4 |
The wild-type and mutant cultures were placed in serum bottles with Teflon-coated stoppers when the aerobic cultures reached an OD at 600 nm of approximately 0.3. Oxygen-limiting conditions were obtained by sparging the bottles with argon gas for 2 min. The cultures were then incubated at 37°C with shaking (200 rpm). Total RNA was isolated after 2 h of induction. No nitrate or nitrite was added.
Data are ratios of the dye intensity of the wild-type strain to that of the mutant strain. Each data point is an average of the results from three independent experiments. A ratio of 2.0 or below was not considered a significant increase.