TABLE 7.
Genes that showed increases in mRNA levels in resDE mutant grown under aerobic conditionsa
| Gene | Description | Induction (fold) |
|---|---|---|
| cydD | 12.0 | |
| cydC | 9.8 | |
| cydB | 5.3 | |
| cydA | 5.4 | |
| yrhG | Similar to formate dehydrogenase | 9.9 |
| yrhE | Similar to formate dehydrogenase | 5.6 |
| yrhD | Unknown | 3.8 |
| lctP | 6.6 | |
| lctE | l-Lactate dehydrogenase | 2.9 |
| alsS | Acetolactate synthase | 2.7 |
| alsD | 2.6 | |
| glpD | Glycerol-3-phosphate (G3P) dehydrogenase | 4.3 |
| mtlD | Mannitol-1-phosphate dehydrogenase | 5.3 |
| yjlC | Unknown | 3.4 |
| yjlD | Similar to NADH dehydrogenase | 3.4 |
a
Mutant and wild-type strains were grown under aerobic conditions to the exponential phase. Total RNAs from these two strains were labeled with either Cy3-dCTP or Cy5-dCTP. The probes were then combined and hybridized to the same slide. The fold induction was the ratio of the dye intensity of the mutant to that of the wild-type strain. (Data are averages from at least three independent experiments.)