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Spheroid fusion assay. (A) Schematic overview of the experimental procedure to obtain spheroid pairs. The main steps are cell seeding, cellular aggregation, differentiation, harvesting/centrifugation, and spheroid seeding. (B) Time-lapse images of the morphological response during spheroid fusion for all different maturation stages of the Y pool. (C) After 48 h, the fused constructs were stained for Alcian Blue (AB) and Collagen Type II (Col2). Scale bars: 50 µm.
