TABLE 3.
Structural parameters for wild-type forespore PG
| Time in sporulation (h) | % Spore PG madea | % Muramic acid with:
|
% Total spore muramic acid with TriPc | ||||
|---|---|---|---|---|---|---|---|
| Side chains of:
|
Cross-link | ||||||
| Lactamb | Ala | TriP | TP | ||||
| 3.5 | 11 | 29.2 | 29.8 | 29.1 | 11.9 | 6.0 | 3.2 |
| 4 | 22 | 39.1 | 33.6 | 13.7 | 13.6 | 3.5 | 3.0 |
| 4.25 | 29 | 42.2 | 31.8 | 9.3 | 16.6 | 2.9 | 2.7 |
| 4.5 | 35 | 43.8 | 30.7 | 7.2 | 18.3 | 2.5 | 2.5 |
| 4.75 | 41 | 44.1 | 30.2 | 6.8 | 18.9 | 2.7 | 2.8 |
| 5 | 47 | 45.2 | 27.9 | 5.3 | 21.6 | 2.7 | 2.5 |
| 5.25 | 53 | 45.5 | 27.0 | 4.7 | 22.7 | 2.9 | 2.5 |
| 5.5 | 60 | 45.5 | 27.0 | 4.2 | 23.3 | 2.8 | 2.5 |
| 5.75 | 65 | 46.4 | 25.5 | 3.7 | 24.4 | 3.1 | 2.4 |
| 6 | 71 | 46.6 | 25.3 | 3.6 | 24.5 | 3.2 | 2.6 |
| 6.25 | 76 | 46.9 | 24.6 | 3.4 | 25.2 | 3.2 | 2.6 |
| 6.5 | 82 | 46.9 | 24.5 | 3.2 | 25.4 | 3.2 | 2.6 |
| 6.75 | 86 | 47.4 | 23.8 | 2.7 | 26.1 | 3.5 | 2.3 |
| 7 | 90 | 47.5 | 23.5 | 2.6 | 26.4 | 3.4 | 2.3 |
| 7.5 | 97 | 47.7 | 23.0 | 2.5 | 26.8 | 3.5 | 2.4 |
| 8 | 100 | 47.7 | 23.2 | 2.4 | 26.6 | 3.4 | 2.4 |
| 24 | 100 | 46.1 | 20.0 | 2.6 | 31.2 | 3.6 | 2.6 |
a
These values are derived from the interpolation of cell muramic acid contents as determined by amino acid analyses.
b
Lactam, muramic-δ-lactam.
c
The amount of muramic acid with a TriP side chain detected in each sample is expressed as a percentage of the total muramic acid found in spore PG at t24.