TABLE 2.

Cma for pHYG3 linker derivatives in matings involving S. lividans strains with circular versus linear chromosomes^a

Plasmid	Chromosome configuration	Relative Cma (%)
pHYG3	Linear	100
	Circular	220
pHYG3-L13	Linear	1.3
	Circular	4.3 × 10−3
pHYG3-L115	Linear	3.1 × 10−1
	Circular	5.1 × 10−2

^aMatings between either S. lividans ZX7 (linear chromosome) or YSC27-14 (circular chromosome), in which each contained the indicated plasmids, and strain TK23(pGSP196) were performed and quantified for Cma as described in Materials and Methods. Numbers represent the averages for two independent matings, in which nearly identical results were obtained.