FIG. 2.

Induction conditions affect levels of cell-bound virulence proteins. Wild-type strain C58 (wt) and virB6 deletion mutant CB1006 (Δ6) were grown on AB minimal medium plates at 20°C (lanes 1), in liquid culture at 20°C (lanes 2), or in liquid culture at 28°C (lanes 3). Cell lysates were subjected to SDS-PAGE followed by Western blotting and detection with specific antisera as indicated. Arrowheads indicate VirB proteins that accumulate to reduced levels in CB1006 compared to wild type only in liquid culture at 28°C. Numbers on the right are molecular masses of reference proteins.