FIG. 3.

Effects of differential expression of VirB6 on other Vir proteins in cell lysates from wild-type strain C58 (lanes 1) and virB6 deletion strain CB1006 carrying cloning vector pTrc200 (lane 2), pTrcB6 (lanes 3), or pTrcTraD (lanes 4) grown on AB minimal medium plates at 20°C in the absence of induction, in the presence of AS for virulence gene induction, or in the presence of AS and IPTG for simultaneous induction of the trc promoter. Cell lysates were subjected to SDS-PAGE followed by Western blotting and detection with specific antisera as indicated. Arrowhead indicates putative degradation products of VirB5 detected in VirB6-overexpressing cells. Numbers on the right are molecular masses of reference proteins in kilodaltons.