TABLE 2.
Rate of synthesis and partial identification of the proteins observed in larger amount in gls24 mutant cells compared to the wild type
| Protein no. | RISa
|
N-terminal sequence | Corresponding gene productb | |||
|---|---|---|---|---|---|---|
|
E. faecalis JH2-2
|
E. faecalis gls24 mutant
|
|||||
| Growing phase | 24 h of starvation | Growing phase | 24 h of starvation | |||
| 1 | UDc | UD | 0.84 | 0.9 (1d) | XXXDFAIE | Lipoamide dehydrogenase |
| 2 | 0.3 | 0.1 (0.3) | 0.96 | 0.37 (0.3) | AAGNKDHQ | l-Lactate dehydrogenase |
| 3 | 0.08 | 0.25 (3) | 0.7 | 1.3 (1.8) | AQKTMIQA | Pyruvate decarboxylase |
| 4 | 0.1 | 0.05 (0.5) | 0.4 | 0.27 (0.6) | XEFLDSLK | Unknown |
| 5 | UD | UD | 0.04 | 0.31 (7.7) | ||
| 6 | 0.4 | 0.13 (0.3) | 0.38 | 0.37 (1) | ||
| 7 | UD | UD | 0.05 | 0.13 (2.2) | XEFQLEKN | Unknown |
| 8 | UD | UD | 0.02 | 0.07 (2.7) | ||
| 9 | UD | UD | 0.03 | 0.52 (14) | XNEGFNNVP | Unknown |
a
Relative intensity of synthesis (RIS) is the ratio of the intensity of each spot to the total intensity on the autoradiograms × 100.
b
Gene product was obtained by comparing the ORF from the sequenced genome of E. faecalis containing the N-terminal sequence and databases. See text for more details.
c
UD, undetectable.
d
RIS after 24 h of starvation/RIS in exponential phase.