(A) No detection of S830 signal in WT control mice.
(B) Nuclear accumulation and aggregation of mHTT in striatum of 12-month-old mice.
(C) mHTT Nuclear aggregates and inclusions in striatum of 18-month-old mice.
(D) Nuclear and cytosolic mHTT accumulation and aggregates in deeper cortical layers of 18-month-old mice.
(E–G) MSD assay for total, expanded, and aggregated mHTT forms in 2- to 18-month-old cortex, striatum, and cerebellum tissues. Results are shown as mean ± SEM, n = 4 for each age groups. *p < 0.05, ***p < 0.001. Scale bars, 20 mm. See also Figure S4