KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Sheep polyclonal anti-mHTT (S830) | Sathasivam K. et al., 2001 | N/A |
| Mouse anti-polyglutamine-expansion diseases marker antibody (clone 5TF1–1C2) | Millipore | Cat# MAB1574; RRID: AB_94263 |
| Mouse monoclonal anti-NeuN (clone A60) | Millipore | Cat# MAB377; RRID: AB_2298772 |
| Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (GFAP) antibody |
Agilent | Cat# N1506; RRID:AB_10013482 |
| Anti-Alpha-actinin-2 / ACTN2 antibody | Abcam | Cat# ab68167; RRID: AB_11157538 |
| Rabbit polyclonal anti-Ubiquitin antibody | Sigma-Aldrich | Cat# U5379, RRID:AB_477667 |
| Mouse anti-Huntingtin protein antibody (clone mEM48) |
Millipore | Cat#MAB5374;RRIDAB_2307353 |
| V5 Tag Monoclonal Antibody | Invitrogen | Cat # R960–25 |
| Ib1 antibody | Wako | Cat#019–19741 |
| Lgals3 antibody | Santa Cruz Biotechnology | Cat# sc-19283 RRID:AB_2136897 |
| Mbnl1 antibody | Abcam | Cat# ab45899 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Paraformaldehyde | Sigma-Aldrich | Cat# P6148 |
| Proteinase K | Sigma-Aldrich | Cat# P6556 |
| Triton X-100 | Sigma Aldrich | Cat# X100 |
| Protease inhibitor cocktail tablets | Roche | Cat# 11873580001 |
| ProLong Gold Antifade Mountant with DAPI | Invitrogen | Cat# P36935 |
| Vector SG Peroxidase Substrate Kit | Vector Lab. | Cat# SK4700 |
| Critical Commercial Assays | ||
| QIAshredder | QIAGEN | Cat# 79656 |
| RNAeasy Plus Micro Kit | QIAGEN | Cat# 74034 |
| TruSeq Stranded mRNA LT Prep Kit | Illumina | Cat# RS-122–2101 |
| Agilent RNA 6000 Pico Chip | Agilent Technologies | Cat# 5067–1513 |
| SYBR™ Green PCR Master Mix | Thermo Fisher Sci. | Cat# 4309155 |
| Deposited Data | ||
| Raw data files for BAC-CAG and BACHD RNA-seq |
This paper | In the process of data deposition at GEO GSE153827 |
| Experimental Models: Organisms/Strains | ||
| Mouse: BAC-CAG | This paper | JAX: 037050 |
| Mouse: BACHD | The Jackson Laboratory | JAX: 008197 |
| Mouse: MORF3 | The Jackson Laboratory | JAX: 035403 |
| Mouse: CaMK2a-CreERT2 | The Jackson laboratory | JAX: 012362 |
| Recombinant DNA | ||
| RP11–866L6 BAC clone | BACPAC Resource |
https://bacpacresources.org/cloneInfoSearch.php |
| pLD53. SC-AB Shuttle Vector | Gong S. et al., 2002 | US Patent No. 6,821,759 (Rockefeller University) |
| pBC-mHTTEx1–140Q | Dr. Bates G. Uni. Col. London | Upon request |
| pLD53. mHTTEx1–135Q | This paper | Upon request |
| BAC-CAG BAC clone | This paper | Upon request |
| WGCNA an R package for weighted correlation network analysis |
University of California at Los Angeles |
http://www.genetics.ucla.edu/labs/horvath/CoexpressionNetwork/ ; RRID:SCR_003302 |
| ImageJ | NIH |
https://imagej.nih.gov/ij/index.html; RRID:SCR_003070 |
| STAR | N/A |
https://github.com/alexdobin/STAR; RRID:SCR_015899 |
| HTSeq | N/A | https://htseq.readthedocs.io/en/release_0.10.0/ ; RRID:SCR_005514 |
| GraphPad Prism 7 | GraphPad Software |
https://www.graphpad.com RRID:SCR_002798 |
| Adobe Photoshop CC19 | Adobe |
https://www.adobe.com; RRID:SCR_014199 |
| GeneMapper v5 | Applied Biosystem | Cat# 4370784 |
| Imaris 9.7 | Oxford Instrument |
http://www.bitplane.com/imaris/imaris RRID:SCR_007370 |