TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference(s) or source |
|---|---|---|
| Strains | ||
| R. leguminosarum | ||
| ANU794 | Smr derivative of R. leguminosarum bv. trifolii strain TA1; Nod+ Fix+ on white and subterranean clovers | (7, 35) |
| ANU437 | pssA::Tn5 derivative of ANU794; Smr Kmr Exo− Nod+ Fix− on white and subterranean clovers | (7, 35) |
| ANU437/pVF18 | ANU437 complemented with pSP329 carrying the pssA gene from R. leguminosarum bv. viciae VF39; Smr Exo+ Nod+ Fix+ on white and subterranean clovers | This study |
| ANU437/pSPRH4 | ANU437 complemented with pSP329 carrying the pssA gene from R. leguminosarum bv. phaseoli; Smr Exo+ Nod+ Fix+ on white and subterranean clovers | This study |
| VF39 | Wild-type R. leguminosarum bv. viciae; Rifr Nod+ Fix+ on P. sativum, V. sativa, and V. faba | (21) |
| VF39 pssA::Tn5 | pssA::Tn5 derivative of VF39; Rifr Exo− Nod− on V. faba | (21) |
| VF39 pssA-Km | Derivative of VF39 containing an insertion of a Kmr cassette within pssA; Rifr Kmr Exo+/− Nod+ Fix+/− on V. faba | (25) |
| VF39 pssA::Tn5/pVF18 | VF39 pssA::Tn5 complemented with pSP329 carrying the pssA gene from R. leguminosarum bv. viciae VF39; Rifr Exo+ Nod+ Fix+ on V. faba | (21) |
| VF39 pssA::Tn5/pSPRH4 | VF39 pssA::Tn5 complemented with pSP329 carrying the pssA gene from R. leguminosarum bv. phaseoli; Rifr Exo+ Nod+ Fix+ on V. faba | This study |
| VF39 pssD::Tn5 | pssD::Tn5 derivative of VF39; Rifr Exo− Nod− on V. faba | (36) |
| VF39 pssE::Tn5 | pssE::Tn5 derivative of VF39; Rifr Exo− Nod− on V. faba | (36) |
| VF39 pssC-Km | pssC-Km derivative of VF39; Rifr Exo+ Nod− on V. faba | (36) |
| E. coli S17-1 | MM294, RP4-2, Tc::Mu-Km::Tn7 chromosomally intergrated | (39) |
| Plasmids | ||
| pSP329 | RK2ori, Tcr; derivative of pTJS75 containing pUC18 cloning site | Gift from S. Lory (37) |
| pSPRH4 | Constructed by cloning pssA gene from R. leguminosarum bv. phaseoli 8002 into pSP329 | This study |
| pVF18 | Constructed by cloning pssA gene from R. leguminosarum bv. viciae VF39 into pSP329 | (21) |
a
Nod+, ability to nodulate; Nod−, inability to nodulate; Fix+, ability to fix nitrogen; Fix−, inability to fix nitrogen; Fix+/−, reduced ability to fix nitrogen; Exo+, ability to produce EPS; Exo−, inability to produce EPS; Exo+/−, reduced production of EPS; Rifr, rifampin resistant; Smr, streptomycin resistant; Kmr, kanamycin resistant; Tcr, tetracycline resistant.