TABLE 2.

Relative differential levels of synthesis of proteins in response to pssA mutation in R. leguminosarum bv. trifolii ANU437

Spota	% Vol in:		Change in synthesis levelb			Mr/pIc
	ANU794	ANU437	2-fold	3-fold	>5-fold
n2	0.031	0.080	+			37,436/6.31
n3	0.015	0.037	+			37,368/6.05
n4	0	0.043				22,147/5.90
n5	0	0.035				22,880/6.09
n6	0	0.024				22,147/6.23
n7	0	0.052				22,880/6.38
n8	0.025	0.082		+		34,000/7.50
n9	0	0.056				16,100/7.00
n11	0.028	0.075	+			17,000/6.90
n12	0.023	0.044	+			42,570/6.32
n13	0.034	0.064	+			50,012/6.39
n15	0.064	0.236		+		34,321/5.15
n16	0	0.063				30,566/5.11
n17	0.149	0.273	+			30,456/4.97
n18	0.013	0.096			+	45,109/4.78
n19	0.048	0.154		+		34,633/4.94
n20	0.010	0.057			+	35,467/5.02
n21	0.012	0.078			+	34,197/5.02
n22	0.004	0.025			+	114,862/4.99
n23	0.006	0.026		+		21,632/5.30
n24	0	0.095				31,408/4.64
n25	0.026	0.068	+			74,480/5.80
n26	0.160	0.062	−			20,939/5.96

^aArbitrary numbers were assigned to proteins which exhibited a change in their relative synthesis levels in response to a Tn5 insertion in the pssA gene of ANU794. 

^bRelative spot volume ratio for a protein from ANU794 and ANU437 of greater (+) or less (−) than two-, three-, or fivefold. 

^cObserved M_r and pI of the protein based on the migration of proteins in the gels.