TABLE 3.

Proteins in the pssA mutant stimulon from R. leguminosarum bv. trifolii ANU437 analyzed by N-terminal microsequencing and FASTA searching

ANU437 mutant-responsive proteins			Characteristics of matching protein from database
Spota	Mr/pI	N-terminal sequenceb	Homologous protein	Organism	Mr/pIc	Swiss-Prot accession no.
n2	37,436/6.31d	QTDAPLLSKV	MigA	P. aeruginosa	34,376/8.31	P95448
		:::::.: :
		TTDAPLVSVV
		13    22
n4	22,147/5.90e	SNDLIVGFSG	Unknown
n5	22,880/6.09e	SAPKLVGLAGSF	Unknown
n6	22,147/6.23e	SNDLIVGFSGNL	Unknown
n7	22,880/6.38e	SAPKLVGLAGSF	Unknown
n8	34,000/7.50d	SNPVLVNQIIPESRVV	Unknown
		[G/T]LT[A/D]SV
n15	34,321/5.15d	ADKKVVVAYQTDALP::::.::: .:  .:	Glutamine-binding periplasmic protein	E. coli	24,963/6.87	P10344
		ADKKLVVATDTAFVP
		22        36
n16	30,566/5.11d	LXXQQKKEFGEFIKQYL	Unknown
n17	30,456/4.97d	EDKSIKVGIMAGEEE	Unknown
n18	45,109/4.78d	DGLSGAPAPFDKGGV	Unknown
n19	34,633/4.94d	TDTVKLRYLASQGNL	Unknown
n20	35,467/5.02d	SILKDDNIDNHADDR	Unknown
n21	34,197/5.02d	ADKKVVVAYQTDALP::::.::: .:  .:	Glutamine-binding periplasmic protein	E. coli	24,963/6.87	P10344
		ADKKLVVATDTAFVP
		22           36
n22	114,862/4.99e	EDKSIKVGIMAGEEEDIXRVVASEAAK	Unknown
n23	21,632/5.30e	WSTFLYT	Unknown

^aArbitrary numbers were assigned to proteins which exhibited a change in their level of synthesis in response to a Tn5-induced mutation in the pssA gene of R. leguminosarum bv. trifolii strain ANU794. These numbers correspond to those in Fig. 3, 4, and 5. 

^bN-terminal sequences are shown by using the single-letter for amino acid residues. A comparison of similar sequences is displayed. Colons indicate identical amino acids, and single dots indicate conserved amino acid substitutions. 

^dThe predicted molecular weights and isoelectric points of the matched sequences were calculated from the amino acid sequence of the protein using the “compute pI/M.m” tool accessed on the ExPasy server (http://www.expasy.ch).