TABLE 4.

Relative differential levels of synthesis of proteins in response to pssA mutation in R. leguminosarum bv. viciae VF39

Spota	% Vol in:		Change in synthesis levelb			Mr/pIc
	VF39	VF39 pssA::Tn5	2-fold	3-fold	>5-fold
v1	0.005	0.011	+			114,000/4.98
v2	0.043	0.181		+		35,178/4.85
v3	0	0.024				26,005/4.74
v4	0	0.100				25,620/4.82
v5	0.013	0.068			+	38,385/5.30
v7	0.074	0.396			+	31,186/5.28
v8	0.335	0.920	+			29,294/5.28
v9	0.086	0.175	+			17,142/5.34
v10	0.007	0.046			+	10,091/5.23
v13	0.033	0.071	+			28,990/5.87
v14	0.057	0.118	+			28,562/5.98
v15	0.028	0.056	+			18,722/5.65
v16	0.060	0				14,093/4.99
v17	0.029	0.111		+		14,793/5.78
v18	0.036	0.091	+			19,047/5.97
v19	0.012	0.042		+		13,500/6.19
v20	0.018	0.127			+	29,557/6.57
v21	0	0.293				28,477/6.62
v22	0.008	0.060			+	14,277/6.48
v23	0.025	0.055	+			13,764/6.67
v24	0.018	0.049	+			11,463/4.83
v25	0.075	0.036	−			28,604/6.53

^aArbitrary numbers are assigned to proteins which exhibited a change in their relative synthesis levels in response to a Tn5 insertion in the pssA gene of VF39. 

^bRelative spot volume ratio for a protein from VF39 and VF39 pssA::Tn5 of greater (+) or less (−) than two-, three-, or fivefold. 

^cObserved M_r and pI of the protein based on the migration of the gels.