TABLE 5.
Comparative analysis of the 22 protein differences associated with VF39 pssA::Tn5 in the pssC, pssD, and pssE mutants and the VF39 pssA::Tn5 transconjugants
| Spot no. | Spot differencea in:
|
|||||
|---|---|---|---|---|---|---|
| VF39 pssA::Tn5b | VF39 pssA::Tn5/pVF18c | VF39 pssA::Tn5/pSPRH4c | VF39 pssD::Tn5c | VF39 pssE::Tn5c | VF39 pssC-Kmc | |
| v1 | Up-reg (+++) | 0 | 0 | 0 | 0 | 0 |
| v2 | Up-reg (+++) | +++ | +++ | +++ | +++ | 0 |
| v3 | Induced (+++) | +++ | +++ | +++ | +++ | 0 |
| v4 | Induced (+++) | +++ | +++ | +++ | +++ | 0 |
| v5 | Up-reg (+++) | 0 | 0 | +++ | +++ | 0 |
| v7 | Up-reg (+++) | +++ | +++ | 0 | 0 | 0 |
| v8 | Up-reg (+++) | +++ | +++ | 0 | 0 | 0 |
| v9 | Up-reg (+++) | 0 | 0 | +++ | +++ | 0 |
| v10 | Induced (+++) | 0 | 0 | 0 | 0 | 0 |
| v13 | Induced (+++) | 0 | 0 | ++ | ++ | 0 |
| v14 | Up-reg (+++) | +++ | +++ | +++ | +++ | 0 |
| v15 | Up-reg (+++) | 0 | 0 | ++ | ++ | 0 |
| v16 | Repressed (−−−) | −−− | −−− | 0 | 0 | 0 |
| v17 | Induced (+++) | +++ | +++ | 0 | 0 | 0 |
| v18 | Up-reg (+++) | 0 | 0 | 0 | 0 | 0 |
| v19 | Up-reg (+++) | +++ | +++ | 0 | 0 | 0 |
| v20 | Up-reg (+++) | +++ | +++ | +++ | +++ | 0 |
| v21 | Induced (+++) | +++ | +++ | 0 | 0 | 0 |
| v22 | Up-reg (+++) | ++ | ++ | 0 | 0 | 0 |
| 23 | Up-reg (+++) | +++ | +++ | 0 | 0 | 0 |
| v24 | Up-reg (+++) | +++ | +++ | 0 | 0 | 0 |
| v25 | Down-reg (−−−) | −−− | −−− | 0 | 0 | 0 |
a
For comparison, the symbols +++ and −−− are assigned to the spot differences in VF39 pssA::Tn5 and represent the maximum degree of up-regulation (up-reg) or repression, respectively. 0 indicates that the synthesis has been restored to a level similar to that of wild-type VF39.
b
Protein differences compared with wild-type VF39.
c
Protein differences compared with VF39 pssA::Tn5.