Figure 4. Tregs preferentially contact CD11c⁺ APCs that are substantially and simultaneously interacting with Teffs.

(A) Heatmap of individual CD11c⁺ APCs and their contacts over time separated according to whether they were contacted by Teffs only (left column) or by both Teffs and Tregs (right column; from representative Teff + Treg movie in Figure 3A). (B and C) Overall mean CD11c-Teff contact indexes and color-coded overall mean contact indexes of CD11c⁺ APCs being contacted by Teffs only or by both Teffs and Tregs (from Teff + Treg movies in Figure 3A). Mann-Whitney test used. n = 3–4 mice per group using 2 or more movies for each mouse. For each movie, an average of 321 CD11c⁺ APCs were analyzed. *P < 0.05.