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. 2022 Aug 22;7(16):e153058. doi: 10.1172/jci.insight.153058

Figure 5. GDF15 neutralization attenuates bleomycin-induced fibrotic gene expression.

Figure 5

(A–E) qPCR analysis of profibrotic gene expression in the lung of bleomycin-treated mice. The impact of GDF15 neutralization on the bleomycin-induced expression of Col3a1 (A), Col1a2 (B), Col1a1 (C), p21 (D), and Ccl2 (E) genes. The expression of the genes of interest was calculated after the normalization to 18S housekeeping gene expression, and the data are presented as 2–ΔΔCT, corresponding to the fold change in gene expression above saline/saline control mouse group. Data are presented as box-and-whisker plots. n = 6–8; *P < 0.05, **P < 0.01, and ***P < 0.001 (analyzed using multiple comparison Kruskal-Wallis test).