Figure 2. The Sema7a^R145W mutation causes intrahepatic accumulation of small lipid droplets in male mice at 10 weeks of age.

(A) Representative images (original magnification, ×200) of H&E staining, Sirius red staining, and Oil Red O staining in WT and Sema7a^R145W heterozygous and homozygous male mice at the age of 10 weeks. (B) Analysis of lipid droplets in the Oil Red O–stained liver sections of WT (n = 6) and Sema7a^R145W heterozygous (n = 9) and homozygous male mice (n = 9). (C) The levels of serum ALT and AST in Sema7a^R145W WT (n = 6), heterozygous (n = 9), and homozygous male mice (n = 9). The data were analyzed by 1-way ANOVA with Tukey’s post hoc tests or by Kruskal-Wallis test with Dunn’s post hoc test analysis.